NewBio RPR (Rapid Plasma Reagin) utilises carbon particles coated with cardiolipin antigen to detect reagin antibodies present in serum or plasma of syphilitic persons.
Specimens that contain reagin cause aggregation of the carbon particles which appear as dark clumps against a white background. The aggregation can be read macroscopically. Non-reactive samples typically appear as a smooth non-aggregated pattern which may form buttons in the centre of the test area.
Description
Non-treponemal carbon antigen test for syphilis
Carbon particles and VDRL antigen
All reagents are ready to use, available with controls
RPR antigen
RPR negative control
RPR positive control
Test cards
Pipstirs
Dispenser bottle and needle
Simple protocol: 1 x 8 minutes incubation
Manual
Requires only 50 µL sample
Specificity
A study with 1248 syphilis negative EDTA plasma samples showed 99.92% specificity (95% confidence interval 99.55-100.0%).
Sensitivity
A study with 158 syphilis positive samples (37 EDTA plasma and 121 sera) showed 98.73% sensitivity (95% confidence interval 95.50-99.85%)
