Collection: Newbio Syphilis Tests
NewBio TPHA Syphilis Test
NewBio TPHA is a passive particle agglutination assay for the qualitative detection of IgG and IgM antibodies to Treponema pallidum in human serum, EDTA plasma, or CSF and to determine the titre level of the samples.
NewBio TPHA uses preserved avian erythrocytes coated with extracted antigens of T.pallidum (Nichols strain). Specific antibodies present in a sample of plasma or serum bind to these antigens when the sample is incubated with the particles. This causes the particles to agglutinate, then settle to form a characteristic pattern in the test well.
Non-specific reactions are eliminated by the use of absorbents. The assay is run and interpreted manually.
Newbio RPR Syphilis Test
NewBio RPR (Rapid Plasma Reagin) utilises carbon particles coated with cardiolipin antigen to detect reagin antibodies present in serum or plasma of syphilitic persons.
Specimens that contain reagin cause aggregation of the carbon particles which appear as dark clumps against a white background. The aggregation can be read macroscopically. Non-reactive samples typically appear as a smooth non-aggregated pattern which may form buttons in the centre of the test area.
